The following guidelines outline the minimum level of care that we expect from researchers studying and borrowing our specimens.
Maintain specimens in 70% ethanol. They should not be transferred to any other concentration of ethanol or any other liquid (including isopropanol).
Keep specimens moist during examination. They should be kept moist with 70% ethanol NOT WATER. Never transfer MCZ specimens to water, not even briefly.
Examine specimens with care to avoid damage. Please do not force limbs, jaws, etc. into positions that tear muscles or break bones.
Any dissection of MCZ specimens (including sex determination) requires the written approval of the MCZ curator and/or collections manager. Permission to modify one specimen is not automatically conferred to other specimens. When in doubt, ask.
When permission to determine sex has been given, incisions must be performed to avoid cutting skeletal elements and viscera; incisions should be kept as small as possible. Anurans: Incise the dorsolateral body wall, adjacent to the sacral diapophysis. Salamanders and lizards: Make a midventral incision. Begin at midbody, posterior to sternal elements (including inscriptional ribs), and extend posteriorly to the level of the pubes, where a lateral incision on one side is permitted. Snakes, amphibaenians, and caecilians: Make a short, mid-ventral incision over the gonad region; do not extend the incision from the vent forward to midbody. If the incision gapes open, close it with 1 or 2 suture stitches. If a snake is sexed by hemipenial dissection, the incision should be made to locate only one hemipenis.
In cases where organs are removed for histological examination, the tissue and the finished microscopic slides should be returned to the MCZ upon completion of the study. Stomach contents are also to be returned. Large items may be returned to the stomach; small or large items returned in vials must be labeled accurately with respect to specimen derivation (MCZ number and determination) and identification of contents.
Specimens that are to be cleared and double-stained should have the following additional data recorded by the preparator: snout-vent length, body and tail length (when appropriate), sex and reproductive condition (if possible). Additionally, the skin and viscera are to be returned, properly labeled with the name and number of that specimen.